asymmetric flow field flow fractionation af4 characterization Search Results


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Postnova Analytics af4
Figure 4. Non-covalent loading of protein on polyphosphazene carriers. (A) Avidin bound to polyphosphazenes as a percent of total protein in formulation and number of protein molecules per polymer chain <t>(AF4</t> analysis, 210 nm, 0.015 mg/mL of polyphosphazene, 0.10 mg/mL of avidin, PBS, pH 7.4, cross- flow 4 mL/min); (B) AF4 profiles of avidin-polyphosphazene-FITC-biotin complexes resulting from (1) avidin-
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PanReac AppliChem af4-uv–vis-dls liquid carrier (0.1 filtered)
Figure 4. Non-covalent loading of protein on polyphosphazene carriers. (A) Avidin bound to polyphosphazenes as a percent of total protein in formulation and number of protein molecules per polymer chain <t>(AF4</t> analysis, 210 nm, 0.015 mg/mL of polyphosphazene, 0.10 mg/mL of avidin, PBS, pH 7.4, cross- flow 4 mL/min); (B) AF4 profiles of avidin-polyphosphazene-FITC-biotin complexes resulting from (1) avidin-
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Figure 4. Non-covalent loading of protein on polyphosphazene carriers. (A) Avidin bound to polyphosphazenes as a percent of total protein in formulation and number of protein molecules per polymer chain (AF4 analysis, 210 nm, 0.015 mg/mL of polyphosphazene, 0.10 mg/mL of avidin, PBS, pH 7.4, cross- flow 4 mL/min); (B) AF4 profiles of avidin-polyphosphazene-FITC-biotin complexes resulting from (1) avidin-

Journal: Biomacromolecules

Article Title: Biodegradable “Smart” Polyphosphazenes with Intrinsic Multifunctionality as Intracellular Protein Delivery Vehicles

doi: 10.1021/acs.biomac.7b00537

Figure Lengend Snippet: Figure 4. Non-covalent loading of protein on polyphosphazene carriers. (A) Avidin bound to polyphosphazenes as a percent of total protein in formulation and number of protein molecules per polymer chain (AF4 analysis, 210 nm, 0.015 mg/mL of polyphosphazene, 0.10 mg/mL of avidin, PBS, pH 7.4, cross- flow 4 mL/min); (B) AF4 profiles of avidin-polyphosphazene-FITC-biotin complexes resulting from (1) avidin-

Article Snippet: Asymmetric Flow Field Flow Fractionation, AF4 was performed using a Postnova AF2000 MT series (Postnova Analytics GmbH, Landsberg, Germany).

Techniques: Avidin-Biotin Assay, Formulation, Polymer